Preventing Cell Death

Preventing Cell Death Ultrapure Water System Lowers Endotoxin Content Below Prescribed Limits Katrin Toeppner1, Dr. Elmar Herbig2 1. Sartorius Stedim GmbH, Goettingen, Germany 2. Sartorius Lab Instruments GmbH & Co. KG, Goettingen, Germany Introduction The cell wall components of gram-negative bacteria, such as E. coli and pseudomonads, are designated as endotoxins. They have a hydrophilic polysaccharide and a lipophilic lipid component and, unlike the bacteria from which they originate, are highly heat and pH-stable. Endotoxins belong to pyrogens, i.e., they can cause fever if they come in contact with mucous membranes or if they enter the bloodstream (with reference to Steck, 2006, [1]). According to the prevalent pharmacopeias, the defined limit values for endotoxin content may not be exceeded during the manufacturing process of pharmaceuticals. In mammalian cell cultures that are used to produce biopharmaceuticals, such as immunoglobulins, the presence of endotoxins can lead to cell death. For this reason, ultrapure media, i.e., ultrapure water, with levels proven to be below the limits must be used to manufacture biopharmaceuticals or to propagate cell lines or cell cultures. The objective of this study is to demonstrate that the ultrapure water produced by the Arium® Pro VF water purification system exhibits an endotoxin content that is far below the prescribed limits and that can be used for applications of the categories mentioned above. June 22, 2021 Keywords or phrases: Ultrapure water, manufacturing of pharmaceuticals, detection of endotoxins, endotoxin concentration, Pharmacopoeia, mammalian cell cultures, production of immunoglobulins, gel-clot method, quantitative chromogenic method Find out more: www.sartorius.com 2 Endotoxin Testing A method for detection of endotoxins is the so-called LAL (Limulus Amebocyte Lysate) test, which uses the clotting reaction of a lysate of amebocytes isolated from the horseshoe crab (Limulus polyphemus). This reaction involves a primitive evolutionary coagulation cascade in which proteases are sequentially activated, thus resulting in the formation of a clot. The coagulation cascade (also called a Limulus Enzyme Cascade, see [2], [3]) can be triggered by endotoxins or alternatively by ß-glucans, which are short-chain polysaccharides found in the cell walls of yeasts and molds. The ß-glucan pathway may lead to false-positive results [1]. Based on coagulation, the liquid, colorless lysate reacts by coagulation to form a solid, milky gel, hence the term “gelclot method.” As the sensitivity of the lysate is extremely high, it is imperative to rule out a falsepositive reaction caused by contamination with endotoxins or ß-glucan. For this reason, exceptionally high purity requirements must be placed on ultrapure water. In addition to the gel-clot method, the endotoxin content can also be assayed using a quantitative chromogenic method. In this method, a synthetic chromogenic peptide, a chromogenic substrate, is added to the lysate. Whereas in the gel-clot method, a gel clot is formed by the clotting enzyme, the synthetic chromogenic substrate in the chromogenic method is split by the clotting enzyme, resulting in a color change to yellow. The intensity of the yellow coloration correlates directly with the endotoxin content of the sample (chromogenic test). Preparing the lysate for either the gel-clot or the chromogenic method must therefore be done using ultrapure water. An Ultrapure Water System The Arium® Pro VF system (Fig. 1) is designed to produce ultrapure water from pretreated drinking water and removes any contaminants still present in this potable water. Ultrapure water production requires continuous recirculation and a constant water flow rate, which is achieved using a pump system with controlled pressure. The conductivity of the water is measured at the feed water inlet and at the downstream port, or product water outlet. The system used in the tests described in this paper operates with two different cartridges. These are filled with a special active carbon adsorber and mixed-bed ion exchange resins to deliver ultrapure water with a low TOC content. Moreover, the unit has an integrated UV lamp that has an oxidizing effect at wavelengths of 185nm and 254nm, respectively. Furthermore, the system has a built-in ultrafilter module used as a crossflow filter. The ultrafilter membrane utilized in this filter retains colloids, microorganisms, endotoxins, RNA and DNA. A 0.2μm final filter installed at the water outlet serves to remove particulates and bacteria during dispensing of the ultrapure water stream. The process that the unit employs to produce ultrapure water is shown in Figure 2. Figure 2: Schematic flow diagram Test Methods In the tests performed to assay the endotoxin content in ultrapure water obtained from the system, both the gel-clot method and the chromogenic method were conducted. Gel-Clot method The lyophilized lysate (Charles River Endosafe R 15006) was dissolved in ultrapure water obtained from the system and frozen by portions in pyrogen-free test tubes. An endotoxin standard series consisting of LPS E. coli 055:B5 (Lonza N 185) was prepared in concentrations of 0.001 EU/mL to 25 EU/mL using ultrapure water from the Arium® Pro VF system. The dissolved lysate was tested using the endotoxin standard prepared in order to verify that it had the sensitivity indicated (0.06 EU/mL). A sample of 100μL was pipetted into 100μL of the lysate and incubated in a water bath at 37 °C. After one hour, each tube was individually removed from the water bath, turned by 180° and assessed for gelclot formation. UV lamp (185|254nm) Conductivity measurement (inlet water) Inlet water input Pump Cartridge 1 Cartridge 2 Reject water outlet Conductivity measurement (product water) Final filter 0.2µm Sanitization port Ultrafilter Product water 3 Sample Endotoxin Standard Prepared [EU/mL]* GelClot Chromogenic Assay [EU/mL]* Arium® Pro VF Ultrapure Water 0 No 0** Endotoxin Standard 0.001 No 0** Endotoxin Standard 0.005 No 0** Endotoxin Standard 0.01 No 0.009 Endotoxin Standard 0.05 No 0.055 Endotoxin Standard 0.1 Yes 0.083 Endotoxin Standard 0.5 Yes 0.63 Endotoxin Standard 1.0 Yes 1.2 Endotoxin Standard 5.0 Yes 4.47 Endotoxin Standard 10 Yes 8.4 Endotoxin Standard 25 Yes 25 * EU/mL, endotoxin unit; 1 EU approximately corresponds to 100 picograms of endotoxin (as a rule of thumb) ** Below the quantifiable detection limit Table 1: Assay of the endotoxin content in Arium® Pro VF ultrapure water and endotoxin standard samples using the gel-clot method and the chromogenic method Figure 4 shows the data collected on endotoxin concentrations in tap water, deionized water (DI water) and Arium® water in comparison with two selected endotoxin standards (0.05 EU/mL and 0.005 EU/mL). Chromogenic method The chromogenic substrate (Charles River Endochrome K R1710K) was dissolved in ultrapure water produced by the water purification system. This chromogenic substrate and the endotoxin standard samples were pipetted into 96 endotoxinfree well plates in a ratio of 1:1. The absorption of each sample was measured continuously at one-minute intervals over one hour at 405nm using a TECAN Safire plate reader at 37 °C. Results In the range of 0.001 EU/mL to 0.05 EU/mL, the gel-clot method showed a negative result; i.e. no gel-clot formation (no endotoxins detected; Table 1). A concentration of ≥ 0.1 EU/mL resulted in the formation of a gel-clot. This result shows that within the detection sensitivity limits of the gel-clot method, no endotoxins were present in the ultrapure water filtered by this particular system. The 60 absorption readings at 405nm (Figure 3) in the chromogenic method yielded curves with different slopes depending on the specific endotoxin content of the standard samples. The times that were needed to reach a specific absorption were determined using a special computer program and used as the basis for extrapolating the samples with an unknown endotoxin content (for values, see Table 1 – chromogenic assay). As the standard samples with the lowest concentrations (0.005 and 0.001 EU/mL) and the Arium® water did not attain the extinction value of 1 required to calculate the endotoxin content, they were assessed as below the detection limit. The values attained with Arium® Pro VF ultrapure water were considerably lower than those plotted for the curve of 0.001 EU/mL. 25 EU/mL 10 EU/mL 5 EU/mL 1 EU/mL 0,5 EU/mL 0,1 EU/mL 0,05 EU/mL 0,01 EU/mL 0,005 EU/mL 0,001 EU/mL Arium® water 3.0 2.5 2.0 1.5 1.0 0.5 0.0 0 600 1200 1800 2400 3000 3600 time [sec] absorption [405nm] Endotoxin standards 25 EU/mL–0,001 EU/mL Figure 3: Absorption readings for endotoxin standard samples over a 60-minute period using the chromogenic method. 0,05 EU/mL 0,005 EU/mL Arium® water tap water DI water 3.0 2.5 2.0 1.5 1.0 0.5 0.0 0 600 1200 1800 2400 3000 3600 time [sec] absorption [405nm] Figure 4: Endotoxin assay of water samples. The endotoxin content in water samples was calculated from the values measured using the endotoxin standards and is shown in Table 2. Sample Endotoxin Concentration [EU/mL]* Arium® Pro VF Ultrapure Water <0.001 DI Water 0.02 Tap Water 25 Table 2: Table showing the endotoxin content in different water samples Germany Sartorius Lab Instruments GmbH & Co. KG Otto-Brenner-Strasse 20 37079 Goettingen Phone +49 551 308 0 USA Sartorius Corporation 565 Johnson Avenue Bohemia, NY 11716 Phone +1 631 254 4249 Toll-free +1 800 635 2906 For further information, visit www.sartorius.com Specifications subject to change without notice. Copyright Sartorius Lab Instruments GmbH & Co. KG. Status: 06 | 2021 In the DI water sample, endotoxins of 0.02 EU/mL were detected, which was below the currently valid limits for WFI (water for injection). In tap water, with 25 EU/mL an exceptionally high endotoxin load was found. This finding was not further analyzed. By contrast, an exceptionally low value of <0.001 EU/mL was measured in ultrapure water obtained from the system, which is far below the usual limits encountered. Discussion The test results show that ultrapure water produced by the Arium® Pro VF system can be readily used as an affordable alternative for preparing samples to be assayed in endotoxin tests as the detectable concentrations of endotoxin in the ultrapure water produced are exceptionally low (<0.001 EU/mL). The results obtained confirm earlier tests in which an endotoxin load of <0.001 EU/mL was found in Arium® ultrapure water [4]. The endotoxin concentration is far below the limits required by the United States Pharmacopeia, which therefore makes this water theoretically suitable for use in the manufacture/ endotoxin monitoring of pharmaceutical products. Examples of such uses are product formulations, diafiltration solutions, chromatography buffers and water for extraction, rinsing and sanitization steps as well as for cell culture solutions. Precisely within the scope of cell culture applications one has to pay attention to contaminations in the various steps of these processes. To maintain control over the effects of endotoxins on such cultures and on the sensitivity of cells, particularly with respect to such endotoxins, the media for cell growth must be demonstrably free of detectable endotoxins (see also [5]). Acknowledgements Our special thanks go to Dr. Stephanie Steck of Rentschler Biotechnologie GmbH