Fragment analysis is a powerful genetic analysis technique with simple, straightforward workflows. This technology can be used for a wide variety of DNA sizing applications and is a flexible and effective technique that allows you to multiplex and analyze multiple samples in parallel.
Download this infographic to discover:
- Fragment analysis using capillary electrophoresis
- Simple workflow and data analysis
- Common fragment analysis applications you can adopt in your lab
Fragment Analysis by capillary electrophoresis Technique used in genetics to separate and size fluorescently labeled DNA fragments. This fragment analysis 4-step workflow is straightforward, from beginning to end. To run fragment analysis on a capillary electrophoresis system fluorescently labeled primers are designed to flank the region of interest. 4-step workflow Capillary electrophoresis system Mg+ Polymerase Bu er 5’ Labeled primers 3’ Unlabeled primers dNTPs dATP dGTP dCTP dTTP DNA extraction Polymerase chain reaction 3’ Un-labeled primer 5’ Labeled primer The DNA fragments you’re analyzing are amplified by polymerase chain reaction (PCR) and run on a thermocycler DNA extraction PCR amplification Capillary electrophoresis Data analysis Smaller fragments Larger fragments Gene A Gene F Gene D Gene E Gene B Gene C Migrates slowly Migrates quickly Laser Fragment analysis Prepare Load Run applied biosystems Fragment analysis is great for identification purposes like authenticating the cell line identity of cultured human cells and mutations in microsatellite regions that are characteristic in some cancers, inherited and infectious diseases Fragment analysis CACACACACA CACA CACA Single-nucleotide polymorphism genotyping ATCGTTGTAGCGCTTAGAA TAGCAACATCGCGAATCTT Now the sample is ready to be loaded onto the Applied Biosystems™ genetic analyzer. Fragment analysis can be used for a wide variety of applications such as short tandem repeat genotyping. Learn more at thermofisher.com/fragmentanalysis Single-Nucleotide Polymorphism genotyping Single-Nucleotide Polymorphism genotyping can
