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With the increasing use of nanotechnology in food and consumer products, there is a need for reliable detection and characterization methods for nanoparticles (NPs) in complex matrices. NPs often interact with each other or with their surroundings leading to aggregation, adhesion to surfaces or dissolution in dispersion solvents. Accurate and precise characterization of metrics such as size, shape, particle mass and number concentration therefore remains a challenging analytical task. Asymmetric flow field flow fractionation (AF4) hyphenated with inductively coupled plasma mass spectrometry (ICP-MS) is a powerful technique for the detection and characterization of inorganic NPs in complex matrices. In order to acquire accurate data the AF4 separation method and settings must be optimized for each new sample matrix and analyte NP combination. Furthermore, additional information obtained by single particle (sp) ICP-MS analysis of collected fractions or an imaging method such as transmission electron microscopy (TEM) are necessary for trouble shooting and for independent verification of results. spICP-MS as stand-alone technique is a powerful screening method for many inorganic NPs. A number of examples where AF4-ICP-MS and single particle ICP-MS were applied for the detection and characterization of NPs in food and biological matrices will be presented including: • Silver NPs in chicken meat • Silicon dioxide NPs in tomato soup • Aluminum-containing NPs in noodles • Gold NPs in cells

Today our host Jen Fournier is in the lab with the dirtiest, grimiest samples. She knows if she tries to run them on LC-MS as-is, she’d degrade the column, get low sensitivity … and have to clean the mass spectrometer. Is there a better way to start with clean samples?

A guide on how to harmonise and standardise methods in food microbiology – a case study will be presented.

This talk highlights novel strategies for the detection of single molecules using multiphase microfluidics.

As many biodetection assays, especially nucleic-acid based tests, require a more or less complex sample preparation protocol, microfluidics is seen as one of the key enabling technologies to realize the integration of these sample prep protocols with the detection scheme on a single device.

In this work, we will present the fundamentals of Foodomics, discussing the role of this new discipline to solve some current and future challenges in food science and nutrition, and showing several Foodomics applications carried out in our laboratory related to food quality and food bioactivity.